FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294223
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.12872588417915615No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13220.10214622982283579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.040.8519671
GTATCAA56250.039.8549351
TCGTATG4400.039.3811240
CTCGTAT4250.038.2828839
GTGGTAT8300.037.9958881
CGTATGC4850.035.7691441
TGGTATC8900.035.434372
TATCAAC65250.034.5153052
TCTCGTA4600.033.06849338
TCAACGC69750.032.534244
ATCAACG70400.032.1364673
CAACGCA70750.031.977495
CTTATAC6250.031.2623561
AACGCAG72900.031.0343936
CCGTCTT5100.030.8022147
TATACAC7050.030.6322063
GCCGTCT5250.029.89860246
ACGAGAC5650.029.71366722
CGCAGAG79400.028.5171228
ACGCAGA79200.028.5059367
Produced by FastQC (version 0.11.5)