FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062478
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC42530.4002906413121025No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29690.2794410801917781No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCAT29490.27755868827401603No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21440.20179241358409306No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC21050.19812174934445703No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20490.19285105197472324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19240.18108610248871038No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18970.17854487339973157No Hit
GCATAGATCAGGGACTTAGGGGCCTTCCCTGGTTGCTGCTGAAACCAGGC17500.16470929280418042No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17420.1639563360370756No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17310.16292102048230647No Hit
GATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA16470.1550149744277058No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCA16080.1513443101880698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15650.14729716756488134No Hit
GTGCAGCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG15170.1427794269622524No Hit
ATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAA14690.13826168635962344No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC14140.13308510858577777No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCAGCATCACTT14010.13186155383923245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.13167331464745624No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAAGTGACGT13480.12687321525716297No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG13470.12677909566127488No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT13450.12659085646949866No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT13290.12508494293528902No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13110.12339079020930316No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13100.12329667061341505No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCA12730.11981424556555526No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12670.11924952799022663No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC12440.11708477728480024No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12060.11350823264105234No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11830.11134348193562596No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAAA11660.10974344880552822No Hit
AGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAACCAGGGAAGGCCCCTAAGTCCCTGATCTATG11580.1089904920384234No Hit
GCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG11570.10889637244253529No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11130.10475511022345874No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11040.10390803386046582No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11040.10390803386046582No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10960.103155077093361No Hit
GTCTGCATCTATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGG10950.10306095749747289No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATTTCCAGCTTGGTCC10870.10230800073036807No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10750.10117856557971083No Hit
GTTTCCCAGGTGCCAGAAGTGACGTCCAGATGACCCAGTCTCCATCCTCA10740.10108444598382273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA3900.047.4463770
GTGGTAT4450.044.005211
GGTATCA14400.043.659411
GTATCAA38950.042.8665051
TATCAAC42750.038.895422
TGGTATC5150.037.356832
ATCAACG46600.035.6819573
CTAACGA3600.035.1960769
TCAACGC47250.035.1910974
CAACGCA48000.034.7128075
ATATTCG400.002624234734.42463729
AACGCAG48850.034.10886
TCGTATG1056.220944E-1032.9548340
GTCGTAT1056.2937033E-1032.92672339
CGTATGC1305.456968E-1231.97874641
CGCAGAG52950.031.5840828
AGAGTAC53350.031.54037911
ACGCAGA53450.031.2886267
CAGAGTA54400.030.99472810
TCGTTCG450.00458873530.71269438
Produced by FastQC (version 0.11.5)