FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062478
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC46060.4335148586606028No Hit
GTATAAAACTTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCAT29330.2760527747398064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27390.25779357313751433No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26670.25101696223357095No Hit
GATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA23410.22033397397404936No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC18430.173462415221774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17780.1673446414890473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16950.15953271503033473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16760.15774444270846077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15220.14325002494169292No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCACACGAACTGTGGCTGCACCA15100.14212058979103567No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15000.14117939383215464No Hit
GCATAGATCAGGGACTTAGGGGCCTTCCCTGGTTGCTGCTGAAACCAGGC14740.13873228433906395No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14220.1338380653528826No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC14180.1334615869693302No Hit
GTGCAGCCACAGTTCGTGTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG13930.13110859707212763No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13920.1310144774762395No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13880.1306379990926871No Hit
GTCCCTGATCTATGCTGCCTCCATTTTGCAAAGTGGGGTCCCATCAAAGT13460.12668497606538676No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12760.12009660435321956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12620.11877893001078611No Hit
GGCCAAAAGTGTACGGGTAAGTTTTATACTGTTGGCAGTAATAAATTGCA12560.11821421243545747No Hit
ATAGGAGACAGAGTCAGCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTTCTTAGCCTGGTTTCAGCAGCAA12530.11793185364779317No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12080.11369647183282854No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCAGCATCACTT11820.11124936233973787No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT11790.11096700355207355No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11410.10739045890832562No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11270.10607278456589218No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC11190.10531982779878736No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG11120.10466099062757064No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11030.10381391426457771No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAAGTGACGT10770.10136680477148703No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10730.10099032638793462No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAAA10730.10099032638793462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4750.046.330091
TAACGAA3300.041.85293270
GGTATCA15900.041.7385181
GTATCAA39850.041.159191
TATCAAC43800.037.5742032
TGGTATC5600.037.4257282
ATCAACG47100.035.0577473
TCAACGC47350.034.698214
CAACGCA48250.034.1722455
AACGCAG49250.033.61766
CTTACCG450.0041869131.29329362
CGCAGAG52950.030.8800648
ACGCAGA53850.030.4912747
AGAGTAC54000.030.27961711
GCAGAGT54650.029.9194779
CAGAGTA54950.029.75613210
CTAACGA3200.029.32758169
AGTACGG36700.027.74322913
GAGTACG37550.027.29524812
CGGGAAT5850.026.39191417
Produced by FastQC (version 0.11.5)