FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510248
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40170.2659828054730084No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33390.22108951642379268No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTCCCCTAGAGCCTGAAGATG27620.1828838707285161No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27090.17937451332496385No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCCTCATCGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCT26160.1732165842960891No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26160.1732165842960891No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAG25120.16633029807025074No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24780.16407901218872661No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24450.1618939405978356No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24010.15898051180998088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.14573765368336855No Hit
GTCCCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAAC20560.13613658154157462No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20480.13560686721651014No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20180.13362043849751828No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCTAGATACA19770.13090565258156275No Hit
GCGTAACAACTGGCCAAGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT17970.11898708026761168No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17920.11865600881444636No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTCTTGGCCAGTTGTTACGCTG17440.11547772286405941No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT16980.11243186549493858No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCCTCATCGCCTGGTA16600.10991572245088224No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16260.10766443656935815No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16090.1065387936285961No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15990.10587665072226547No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15620.10342672196884221No Hit
GACTTCACTCTCACCATCAGTCCCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAACAACTGG15350.10163893612174955No Hit
GTTTATTACTGTCAGCAGCGTAACAACTGGCCAAGACTCACTTTCGGCGG15290.10124165037795117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)