FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003454
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22420.22342827872528287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44400.041.3934331
GGTATCA17900.038.6470381
TATCAAC49850.036.5680732
GTGGTAT6600.035.9814221
ATCAACG51350.035.2707673
TCAACGC51700.035.0792274
CAACGCA52950.034.247695
AACGCAG54500.033.3366936
ACGCAGA58400.031.1692497
TGGTATC7650.030.5730552
CGCAGAG59550.030.5673268
AGAGTAC61000.030.17854311
CAGAGTA62900.029.32155410
GCAGAGT63250.028.9420999
GAGTACG36700.026.20456312
AGTACGG36750.026.07675213
GAGTACT34800.025.16783712
AGTACTT34750.025.0076213
GTACTTT36500.023.80862214
TACGGGG15950.022.6140615
Produced by FastQC (version 0.11.5)