FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676509
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31170.46074775058424944No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23240.3435283196528058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19530.288687955370882No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19420.28706196074257695No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17490.25853314590049803No Hit
CTGTTATAGTTTTGACAGTAATAAGTGGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC16460.24330792347182373No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16020.23680394495860366No Hit
GATCTATGGTGCATCCACTTTGCAATCAGGGGTCCCGTCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA15760.23296068492806452No Hit
CTATAACAGTGCCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT15590.23044778413886585No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15130.22364817023868122No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14640.2164051032580498No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14610.21596165017760297No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.2093098539709006No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCGGGGCATAGACAATTATTTAGCCTGGTATCAGCAGAAA13750.20324932853812736No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG13450.19881479773365915No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13100.1936411784617795No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12700.18772847072248852No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT12670.18728501764204172No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12570.18580684070721898No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12400.1832939399180203No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11650.17220761290684974No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG11570.17102507135899153No Hit
GTTATAGTTTTGACAGTAATAAGTGGCAACATCTTCAGGCTGCAGGCTGC11490.16984252981113332No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCC11420.16880780595675743No Hit
ATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTG11410.16865998826327513No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11360.16792089979586375No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11360.16792089979586375No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11040.16319073360443098No Hit
AACTATAACAGTGCCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT10910.1612691035891614No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10620.15698239047817547No Hit
GTGCATCCACTTTGCAATCAGGGGTCCCGTCTCGGTTCAGTGGCAGTGGA10510.15535639584987043No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10480.1549129427694236No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT10300.15225222428674268No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10210.15092186504540223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10030.14826114656272127No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCCACTTATTACTGTCAAAACTATAACAGTGCC10000.14781769348227444No Hit
GTATCAACGCAGAGTACGGGGCAGAAGTCTCTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCT9820.14515697499959349No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9740.1439744334517353No Hit
GTAATAAGTGGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9740.1439744334517353No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9730.14382661575825303No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG9460.13983553803423163No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9280.1371748195515507No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATAGGGCA8920.1318533825861888No Hit
CCATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC8850.1308186587318129No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8640.1277144871686851No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATGTTGCCACTTATTACTGTCAAAACTATAACAGTGCCCTATTCACTTT8320.12298432097725233No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8250.12194959712287642No Hit
GTGGATGCACCATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAATAATTGTCTATG8190.12106269096198276No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGCAGATACCAGAGGTGA7960.11766288401189047No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCGGG7920.11707161323796136No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7870.11633252477054998No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7820.11559343630313862No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7700.11381962398135131No Hit
ACTTATTACTGTCAAAACTATAACAGTGCCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA7700.11381962398135131No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGCAGATAC7650.11308053551393996No Hit
AAAGTGAATAGGGCACTGTTATAGTTTTGACAGTAATAAGTGGCAACATC7600.11234144704652857No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7320.1082025516290249No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7260.10731564546813124No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGACGGGACC7220.10672437469420215No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7160.1058374685333085No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7060.10435929159848575No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7030.10391583851803894No Hit
GCCTGAAGATGTTGCCACTTATTACTGTCAAAACTATAACAGTGCCCTAT6840.1011073023418757No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATG6840.1011073023418757No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6820.10081166695491117No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6800.10051603156794663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3200.050.4128651
GTATCAA30650.044.010431
TATCAAC35800.037.5584032
ATCAACG37350.035.6034163
TCAACGC37650.035.397664
CAACGCA38050.035.1026085
AACGCAG38950.034.3768926
CGCAGAG41600.032.0223778
ACGCAGA42450.031.4618477
AGAGTAC42800.031.28457811
GCAGAGT43050.030.943819
CAGAGTA43500.030.623710
AGTCGGA450.00476102530.48055821
CGGGATC902.1160304E-730.45801717
TCTATTC605.633425E-428.5607283
ATCGTCA500.007194534428.01088155
GAGTACT20000.027.73845912
AGTACGG28450.027.56452613
CGTTTCA500.00790558527.47520628
CGAATTA500.0080061727.4041115
Produced by FastQC (version 0.11.5)