FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390052
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51610.3712810743770737No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36540.2628678639360254No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT23840.17150437537588523No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22090.15891491829082655No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20960.15078572600161722No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20840.14992244894435602No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20730.14913111164186663No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20000.14387950954352788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19710.14179325665514672No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18280.13150587172278447No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18120.13035483564643624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17950.12913185981531627No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17880.1286282815319139No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17170.12352055894311867No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGCCAACTGGCTGCTCACTTTCGGCGGAGGGA16020.11524748714436582No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15720.1130892945012129No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT15000.10790963215764589No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGTCCCTGTT14910.10726217436470002No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG14230.10237027104022009No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACCGAGGTCAGCTTCAGGGAGA14190.10208251202113303No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14180.10201057226636126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53150.041.692691
GGTATCA20250.041.2272871
TATCAAC60150.037.0531122
TCAACGC64500.034.7158554
ATCAACG64600.034.6883283
CAACGCA66800.033.6135375
AACGCAG67900.033.1170926
TGGTATC10900.032.1358152
GTGGTAT11050.031.402381
CGCAGAG72700.030.9305448
AGAGTAC73750.030.4890811
GCAGAGT75200.029.9022679
ACGCAGA75600.029.7440577
CAGAGTA78050.028.89822410
CGTAATT1259.278541E-426.22359870
GAGTACG49850.026.12983112
AGTACGG49850.025.858513
CAGTTCG17500.025.84897470
AATAGCG1251.0266558E-725.38152562
GAGTACT38000.024.89680712
Produced by FastQC (version 0.11.5)