FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034337
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63340.31135451009346043No Hit
GTATGGTAGTCCACCGGAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT54690.26883451463548075No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA48470.238259442757026No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTGGAGCCTGGAGATT42580.2093065209943092No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39000.19170865004175808No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38980.19161033791353152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38600.18974240747722723No Hit
CTACCATACTGCTGACAGTAATAAACTGCAAAATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTC37730.18546582989937263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37000.18187743721910382No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCG35680.175388836756152No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34450.16934264087021964No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGAAACTTCTTAGCCTGGTACC33790.16609834063874374No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCGCT33410.16423041020243942No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32290.15872493102175303No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC31220.153465232161633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30380.1493361227761182No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCT30200.14845131362207933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30030.14761566053215372No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCCTCTGCTGGTACCAGGCTAAGAAGTTTCTGTTAACACTCTGA28720.14117621613331516No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC28260.1389150371841047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27070.13306546555462542No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25660.12613446051465416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25030.12303762847551807No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC24920.12249691177027208No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA24300.11944923579524927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23390.11497603396094158No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA23000.11305894746052399No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT22260.10942139871614191No Hit
GGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCAGCAGGGCC21640.10637372274111909No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21630.10632456667700582No Hit
GTACCAGCAGAGGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGA21330.10484988475360768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21210.10426001198424843No Hit
GTTTATTACTGTCAGCAGTATGGTAGTCCACCGGAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20950.10298195431730339No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20580.10116317994511234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)