FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034337
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60520.2974925000135179No Hit
GTATGGTAGTCCACCGGAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT51080.2510891754905898No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA44460.21854786104760418No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43570.21417297134152308No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTGGAGCCTGGAGATT39220.19279008345225004No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37740.1855149859634859No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36630.18005866284691277No Hit
CTACCATACTGCTGACAGTAATAAACTGCAAAATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTC36030.1771092990001165No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34690.17052238640893816No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCG31510.15489075802091787No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31240.15356354428985955No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31150.1531211397128401No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGAAACTTCTTAGCCTGGTACC30330.14909034245555186No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC30100.1479597529809466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29720.1460918225446423No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCGCT28390.13955406601757725No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25980.12770745456627883No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCT25050.12313594060374461No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24950.1226443799626119No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC24420.12003910856460852No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24350.11969501611581562No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT23990.11792539780773785No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCCTCTGCTGGTACCAGGCTAAGAAGTTTCTGTTAACACTCTGA23860.11728636897426534No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC22440.1103062078701808No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22330.10976549116493482No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA22330.10976549116493482No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21730.10681612731813855No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21650.10642287880523237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21350.10494819688183422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8800.047.2882581
GTATCAA77300.042.310841
GGTATCA28650.042.1340371
TGGTATC10400.041.3104442
TATCAAC87800.037.110522
TCAACGC92550.035.0099144
ATCAACG92800.034.976673
CAACGCA95100.034.206285
AACGCAG96600.033.672646
CGCAGAG103300.031.488658
GCGACTT850.0042312931.22599270
AGAGTAC105750.030.85646211
ACGCAGA105550.030.8174087
GCAGAGT107300.030.2828169
CAGAGTA110200.029.54817210
GAGTACT50700.027.54434612
AGTACTT52050.026.76467513
ACGGGTA3200.025.7434916
GAGTACG73450.025.36612112
AGTACGG73150.025.33005513
Produced by FastQC (version 0.11.5)