FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539840
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21800.4038233550681684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20810.3854845880260818No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT20110.3725177830468287No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18930.3506594546532306No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG18610.3447317723770006No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA18230.3376926496739775No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAAAGT17470.32361440426793125No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGGGTTGTAG16020.2967545939537641No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC15720.2911973918197985No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15450.2861959098992294No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC15320.28378778897451096No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC14530.26915382335506816No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14370.26618998221695317No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14180.2626704208654416No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGTTCATAT14020.2597065797273266No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGGGTTGTAGG13900.25748369887374034No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13610.25211173681090693No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12450.23062388855957322No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACATCA12390.2295124481327801No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAGGGGTTGTAGGTGCCGGCATATGAA12030.22284380557202133No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11660.21598992294013042No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11650.21580468286899823No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11380.21080320094842916No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11260.20858032009484292No Hit
GTACCAACATCACCCAGGCAAAGTCCCCAAACTCATCATTTATGACGTCA10510.19468731475992887No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10400.19264967397747482No Hit
GTCCCCAAACTCATCATTTATGACGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT10330.19135299347954948No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT10000.1852400711321873No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9460.17523710729104922No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9250.17134706579727327No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9160.1696799051570836No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8860.16412270302311796No Hit
GGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAAAGTCCCCAAACTCATCATTTATGACGTCATTAAG8550.15838026081802015No Hit
GATTATTACTGCAGTTCATATGCCGGCACCTACAACCCCTATGTCTTCGG8540.15819502074688796No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC8480.15708358032009484No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC8460.15671310017783047No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8330.15430497925311204No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT7990.14800681683461767No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7980.14782157676348548No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA7880.1459691760521636No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT7840.14522821576763487No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7790.14430201541197393No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7720.1430053349140486No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7680.14226437462951985No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA7620.14115293420272673No Hit
GTATCAACGCAGAGTACGGGGGGGTCTCAGGAGGCAGCGCTCTCAGGACGTCACCACCATGGCCTGGGCTCTGCT7280.13485477178423236No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG7170.1328171310017783No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7170.1328171310017783No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA7130.13207617071724956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.1298532898636633No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7010.1298532898636633No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG6980.12929756965026673No Hit
CCTACAACCCCTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA6940.12855660936573798No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGTTCATATGCCGGCACCTACAA6900.12781564908120924No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA6770.1254075281564908No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6670.12355512744516893No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC6560.12151748666271488No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC6550.12133224659158269No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6550.12133224659158269No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT6510.12059128630705394No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT6490.12022080616478957No Hit
ACATCACCCAGGCAAAGTCCCCAAACTCATCATTTATGACGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTT6450.11947984588026082No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG6420.11892412566686425No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC6410.11873888559573206No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6370.11799792531120332No Hit
GGATGAGGCTGATTATTACTGCAGTTCATATGCCGGCACCTACAACCCCTATGTCTTCGGAAGTGGGACCAAGGT6320.11707172495554238No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG6290.11651600474214582No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6240.11558980438648489No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6200.11484884410195614No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC6140.11373740367516302No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGA6130.11355216360403084No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6070.11244072317723769No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG5980.11077356253704801No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA5890.10910640189685833No Hit
CCCCTGAGGGCCGCTTAATGACGTCATAAATGATGAGTTTGGGGACTTTGCCTGGGTGATGTTGGTACCAGGAGA5810.10762448132780084No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC5650.10466064018968584No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5630.10429016004742146No Hit
CGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC5580.10336395969176053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG200.007415794351.645782
CCGGTGT200.007478595751.535759
ACGGGTG1000.044.67673516
GTATCAA19900.043.6409651
GGTATCA8150.043.553851
GTGGTAT1900.041.7177771
TACGGGT1950.040.53510315
TATCAAC22200.039.3934752
ATCAACG23350.037.288573
TCAACGC23600.036.855954
CAACGCA24050.036.7143175
AACGCAG24500.036.1802026
CACTTGC400.002608986334.462511
CGCAGAG26650.033.261358
AGTACGG13500.033.08468213
ACGCAGA26900.032.9522327
AGAGTAC26650.032.8745911
GCAGAGT26950.032.7636079
GAGTACG13800.032.61441412
CAGAGTA27300.032.34356310
Produced by FastQC (version 0.11.5)