FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023416
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18030.17617469337981817No Hit
GTCTCTGGTGGCTCCATCAGTAAATTCTACTGGAGCTGGATCCGGCAGCC17070.1667943436491124No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16800.16415612028735138No Hit
GTATTACTGTCAGCAGTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGA16610.16229959273648253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15170.14822906814042383No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15110.1476427962822547No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14810.14471143699140915No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14790.14451601303868614No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14770.14432058908596307No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA14610.14275719746417878No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14480.14148694177147905No Hit
CAGTAATACACTGCAAAATCTTCCGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13080.12780726508086643No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11970.11696123570473786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.11393216443753079No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11630.11363902850844623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11620.11354131653208471No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11400.1113916530521313No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCGGAAGATT11010.10758088597403208No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10920.10670147818677839No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10800.10552893447044018No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10500.10259757517959461No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGGTTGAGGGAGA10500.10259757517959461No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAACTTCTTGGCCTGGTACC10240.10005706379419513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)