FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023416
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20980.20499972640646622No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19980.19522852877031432No Hit
GTCTCTGGTGGCTCCATCAGTAAATTCTACTGGAGCTGGATCCGGCAGCC16820.16435154424007442No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16820.16435154424007442No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16400.16024764123289062No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15410.15057415557310028No Hit
GTATTACTGTCAGCAGTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGA14890.1454931328023013No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA13260.12956608065537378No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13020.1272209932226973No Hit
CAGTAATACACTGCAAAATCTTCCGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12410.12126056266464469No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12280.11999030697194493No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12040.11764521953926849No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11170.10914427759581635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11040.10787402190311661No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11030.1077763099267551No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10340.10103418355781031No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA10310.10074104762872575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5700.043.4930081
GTATCAA38550.043.4083861
TGGTATC5850.042.9411352
GGTATCA13750.041.067941
TATCAAC43550.038.4020732
ATCAACG47350.035.372073
TCAACGC48350.034.4796684
CAACGCA48800.034.2053955
AACGCAG49500.033.7200366
CGCAGAG53600.031.3970098
AGAGTAC54550.031.1005411
GCAGAGT55400.030.4388799
CAGAGTA56300.030.07429510
ACGCAGA56500.029.6639087
AGTACGG39700.027.2519913
GAGTACG40300.027.09928312
GAGTACT22950.026.48660712
AGTACTT23200.025.90766313
TTCGGGG8750.025.4816670
TACGGGA21000.024.37537415
Produced by FastQC (version 0.11.5)