FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72303
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5410.748240045364646No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT4930.6818527585300749No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.4716263502205994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.35129939283293915No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2110.29182744837696917No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1910.2641660788625645No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1840.25448459953252284No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1780.24618618867820147No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT1570.2171417506880766No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1510.20884333983375516No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT1480.20469413440659445RNA PCR Primer, Index 29 (95% over 23bp)
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC1440.19916186050371354No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.18256503879507074No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1320.18256503879507074No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC1260.17426662794074935No Hit
GTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAA1140.15766980623210655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1140.15766980623210655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.15628673775638632No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT1130.15628673775638632No Hit
GGTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGA1040.1438391214749042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.14245605299918399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.14107298452346376No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC980.13554071062058282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG970.1341576421448626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.13277457366914236No Hit
GGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAA950.1313915051934221No Hit
GAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCC940.1300084367177019No Hit
TCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCC870.12032695738766026No Hit
TCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGTCTTT790.1092624095818984No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT730.100963998727577RNA PCR Primer, Index 29 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG201.2844717E-463.4183961
CGCCATT150.003278324863.41839616
AGGGGGG1400.061.21695770
GTGGTAT1450.054.671031
CAACAAC200.00700610952.34189240
CTTATAC1650.048.044241
TTATACA1700.046.6311762
TATACAC1700.044.7659263
GGTATCA7450.037.0295331
AAGGGGG1551.4551915E-1136.43871769
CGGGGGG550.002794007333.9232468
GCGTGAA450.002839851433.82314353
GTATCAA14750.033.7514841
TCGTATG1450.033.69133440
ATACACA2300.033.0878564
GCCGTCT1450.032.88659346
TACACAT2350.032.383865
CTCGTAT1400.032.01232539
CGAGACT2650.031.19721223
GAGACTG2550.031.19531824
Produced by FastQC (version 0.11.5)