FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72303
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22053.0496659889631137No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC4670.6458929781613488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.48130782955064105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.35544859826009984No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.2987427907555703No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG2000.27661369514404655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.2531015310568026No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1700.2351216408724396No Hit
GTATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA1650.22820629849383844No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG1530.21160947678519562No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1520.21022640830947542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1470.20331106593087425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.20331106593087425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.1797989018436303No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1300.1797989018436303No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC1290.17841583336791006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.1715004909893089No Hit
GAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCC1110.15352060080494587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.15352060080494587No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1100.1521375323292256No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC1060.1466052584263447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1030.14245605299918399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1020.14107298452346376No Hit
GGTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGA990.13692377909630307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG960.13277457366914236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG930.12862536824198167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG880.12171002586338049No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT870.12032695738766026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA810.11202854653333888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG740.10234706720329723No Hit
GTGCTGCCAAGGGCATCAAGACGATGCTGGTATCACTTCCCTATAGTGAG740.10234706720329723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA730.100963998727577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGT151.2291266E-4144.6936570
CCTGTAT158.722175E-488.5019467
ATCCGGG150.001149415482.5697563
GCATCCG150.001234467481.1005361
AGGCATC150.001314951979.82223559
TTCTGGT150.00226145969.6386541
CTGTATT200.002306418769.26823468
GCACGTG250.003589867861.94133869
CACTGCT253.9846895E-450.43264411
GCTGATA200.00956208148.3848230
CCTCACC200.00969659248.2142129
GGCATCC250.00977477248.1039660
GGTGCAA250.00994590247.89334559
GTGGTAT2250.045.01581
CCACTGC309.785689E-442.027210
GTATCAA18450.036.1979181
TACCACT350.002087459736.0233158
ACCACTG350.002087459736.0233159
GGTATCA8750.032.9178051
TATCAAC21000.031.6297722
Produced by FastQC (version 0.11.5)