FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137046
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49980.439560052979387No Hit
GTCTAACACTTTCCCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46260.4068436984959272No Hit
GTGTTAGACTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC42920.37746933721239073No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35020.3079910575297745No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTTTAGCGGCAGTGGGTCTGGGACAGATTTCA34830.3063200609298129No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33600.29550255662479796No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33480.294447190351138No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33170.29172082747751626No Hit
GGATATTAACAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGCATC29500.25944420894141484No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27840.24484497548911832No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26740.23517078464723504No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26490.2329721049104434No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25880.22760732635267172No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25820.22707964321584173No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGTCAGGATATTAACAGGTGGTTGGCCTGGTATCAGCAGAAA25670.22576043537376675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24100.2119527266267152No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC23900.21019378283728185No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23770.20905046937415023No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGT23490.2065879480689436No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23030.20254237735324693No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGTCTAACACTTTCCCCCCGACGTT22600.19876064820596528No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21710.190933348342987No Hit
GTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTT20470.18002789684850043No Hit
ATTGTCAACAGTCTAACACTTTCCCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG19940.1753666958065021No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19720.17343185763812546No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18890.16613224091197717No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18190.15997593764896056No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17890.15733752196481057No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17580.15461115909118892No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17450.15346784562805726No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGTCTAACACTTTC17230.1515330074596806No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17200.1512691658912656No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17040.14986201085971895No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16690.14678385922821063No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16430.14449723230194733No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16370.14396954916511734No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16090.14150702785991068No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15930.14009987282836403No Hit
GTGGGGTCCCATCCAGGTTTAGCGGCAGTGGGTCTGGGACAGATTTCACT15520.13649403806002572No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15400.1354386717863657No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15150.13323999204957407No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14950.13148104826014073No Hit
TCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGTCT14820.1303377347970091No Hit
GTTAATATCCTGACTCGCCCGACAAGTGATGATGACTCTGTCTCCTACAG14820.1303377347970091No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTGTTA14740.12963415728123576No Hit
AGTCAGGATATTAACAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAA14660.12893057976546243No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14640.1287546853865191No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14450.12708368878655746No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14410.12673190002867077No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14180.12470911467082246No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14130.12426937872346414No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13730.12075149114459749No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCAT13710.12057559676565417No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13610.1196961248709375No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13560.11925638892357918No Hit
CCCCTAAGGTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCA13530.11899254735516418No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13340.11732155075520252No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13280.11679386761837252No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13200.1160902901025992No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13110.1152987653973542No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCA13070.11494697663946754No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13040.11468313507105253No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12880.11327598003950587No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12700.11169293062901588No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12670.11142908906060088No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12530.11019782840799756No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTT12520.1101098812185259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12450.10949425089222424No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12370.1087906733764509No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12160.10694378239754591No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC11890.10456920828181093No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11470.10087542632400096No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11450.10069953194505764No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11390.10017184880822763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT200.00738272851.70789336
GTGGTAT3400.046.4784741
GTATCAA39050.040.907681
ATCGGCG350.001285028639.81296552
CTCGTAT557.346098E-637.6722539
TATCAAC44350.036.1739732
TCGTATG807.0022E-834.55122840
ATCAACG48150.033.3919373
TCAACGC48200.033.35734
CAACGCA49650.032.5907025
CTCGACG2050.031.87081328
AACGCAG50850.031.7540386
CGACCAA450.004702555530.55943525
ACGAGAC2250.030.54867622
ACTCGAC2150.030.38577827
GACTCGA2150.030.383126
CGCAGAG54150.029.811028
TCGACGT2250.029.04424929
AGAGTAC55400.028.95112811
GCAGAGT56450.028.6572449
Produced by FastQC (version 0.11.5)