FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137046
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACTTTCCCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT49220.4328760665795403No Hit
GTGTTAGACTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC49120.43199659468482365No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47610.4187165690746021No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32250.28362968604612304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31610.2780010659199364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29250.25724552920462324No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTTTAGCGGCAGTGGGTCTGGGACAGATTTCA28050.2466918664680233No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25950.22822295667897344No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25610.22523275223693676No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24290.21362372322667683No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23680.2082589446689052No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22500.19788117631124863No Hit
GGATATTAACAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGCATC22490.19779322912177694No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGT22140.19471507749026865No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22110.19445123592185365No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21950.19304408089030697No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGTCTAACACTTTCCCCCCGACGTT21650.190405665206157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20770.1826663125326504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20740.1824024709642354No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCGAGTCAGGATATTAACAGGTGGTTGGCCTGGTATCAGCAGAAA19750.17369569920654046No Hit
GTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTT19180.1686827094066555No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC19010.16718760718563716No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18800.16534071620673219No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18740.1648130330699022No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18690.16437329712254387No Hit
ATTGTCAACAGTCTAACACTTTCCCCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG18660.16410945555412887No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17810.15663394444903725No Hit
GTGGGGTCCCATCCAGGTTTAGCGGCAGTGGGTCTGGGACAGATTTCACT17730.15593036693326393No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17150.15082942994390727No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17100.15038969399654895No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16620.14616822890190898No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16580.1458164401440223No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16230.14273828851251402No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16230.14273828851251402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16190.14238649975462733No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGTCTAACACTTTC15900.13983603125994903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15840.13930834812311904No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT15590.13710966838632738No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15330.13482304146006407No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15120.13297615048115907No Hit
GTCCCAGACCCACTGCCGCTAAACCTGGATGGGACCCCACTTTGCAAACT14700.1292823685233491No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14690.12919442133387743No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14670.1290185269549341No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14560.12805110787074578No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14360.12629216408131244No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCA13910.12233454055508748No Hit
TCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGTCT13510.11881665297622084No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13480.11855281140780584No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13420.11802512827097583No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13400.11784923389203251No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12980.11415545193422254No Hit
GTTAATATCCTGACTCGCCCGACAAGTGATGATGACTCTGTCTCCTACAG12620.11098935311324257No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12570.11054961716588424No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12200.10729557115543259No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12080.10624020488177259No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12050.1059763633133576No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTGTTA12040.10588841612388593No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12000.10553662736599928No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12000.10553662736599928No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11770.10351384200815095No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11550.1015790038397743No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC11510.10122721508188763No Hit
AGTCAGGATATTAACAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAA11460.1007874791345293No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11440.10061158475558596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4400.048.3175771
GTATCAA40000.042.1765751
TATCAAC46000.036.2801742
ATCAACG48350.034.5046543
TCAACGC48650.034.191354
CAACGCA49650.033.6198585
AACGCAG50550.033.156626
CGCAGAG53300.031.4459138
AGAGTAC54350.030.71253211
GCAGAGT55200.030.4254999
CAGAGTA55200.030.36353310
ACGCAGA58450.028.6167097
CTATACC605.680608E-428.5221044
GAGTACT26400.027.85671412
GCATTAC500.007968890527.4319171
TGGATTG5900.026.5356370
CGGGATA2550.025.51109717
AGTACGG37100.025.07895513
GAGTACG37150.024.95202612
TACTTTT29450.024.86979315
Produced by FastQC (version 0.11.5)