FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911022
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46590.5114036763107807No Hit
CTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG40790.4477389130010032No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT40560.44521427583527073No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG32920.36135241519963296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30740.33742324554182007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27990.30723736638632215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27630.30328576038778426No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA27290.2995536880558318No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27140.29790718555644097No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25130.2758440520646044No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24640.2704654772332611No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24510.2690385084004558No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGT24490.26881897473387034No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA22760.2498293125742298No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22710.2492804784077662No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22240.2441214372430084No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA21900.24038936491105595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21470.23566939107946902No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA21050.2310591840811748No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20930.22974198208166213No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20630.22644897708288056No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19640.2155820605869013No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG18450.20251980742506764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18070.1983486677599443No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCC17990.19747053309360257No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17800.1953849632610409No Hit
TTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGA17610.1932993934284792No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17560.19275055926201562No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17130.1880305854304287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16720.18353014526542719No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACC16720.18353014526542719No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16520.1813348085995728No Hit
CTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAG16450.18056644076652376No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16440.18045667393323103No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16390.17990783976676744No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16180.17760273626762033No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15480.16991905793712997No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC15130.16607721877188478No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14860.16311351427298135No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA14760.16201584594005414No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCGAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA14310.15707633844188176No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14130.1551005354426128No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14000.15367356660980744No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGT13870.15224659777700209No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13850.15202706411041664No Hit
AGTCAGAGCCTCCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCA13700.15038056161102584No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13630.1496121937779768No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13590.14917312644480593No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCC13330.14631918877919523No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13160.144453152613219No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12950.1421480491140719No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12820.14072108028126654No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12780.14028201294809564No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12710.1395136451150466No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12700.13940387828175388No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC12560.13786714261565583No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12550.1377573757823631No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGA12110.13292763511748343No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG11820.12974439695199458No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11680.1282076612858965No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11600.12732952661955474No Hit
CTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCT11430.12546349045357852No Hit
GTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCC11340.12447558895394403No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11320.12425605528735859No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11260.12359745428760227No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11000.12074351662199156No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10740.11788957895638086No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10530.11558447545723374No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC10480.11503564129077015No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10440.11459657395759927No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10420.11437704029101384No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10330.11338913879137935No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT10240.11240123729174488No Hit
GTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTC10210.11207193679186672No Hit
CCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT9800.10757149662686522No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9740.10691289562710889No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9710.10658359512723074No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9640.1058152272941817No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9590.1052663931277181No Hit
GTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA9370.10285152279527827No Hit
GCTCCTGATCTATTCGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT9350.10263198912869283No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG9170.10065618612942388No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCTTCCATTAGTA9120.10010735196296026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)