FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229068
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26070.21211194173145828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.11846374651361845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.041.7901271
GTGGTAT8650.041.7413331
GTATCAA62000.041.2088281
TGGTATC9950.037.3244862
TATCAAC71000.035.8398782
ATCAACG75050.034.0432663
TCAACGC75050.033.951634
CAACGCA76000.033.5272375
CGATTGT1855.820766E-1033.16278570
AACGCAG78650.032.3538636
ACGCAGA83750.030.373777
CGCAGAG85300.029.7815428
AGAGTAC88250.028.70810711
CAGAGTA88700.028.60121510
GCAGAGT90050.028.1724389
TCGATTG1705.0749804E-1026.91469
GAGTACG49250.025.19722712
TTATACG1651.1459633E-1025.09034535
AGTACGG49750.025.01308413
ACGAGAC3050.024.80165322
Produced by FastQC (version 0.11.5)