FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229068
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26890.21878366371917585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.12464729372174688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8250.047.0713841
GTATCAA68550.041.309721
GGTATCA26600.040.8261031
TGGTATC9900.038.860012
TATCAAC76850.036.7854422
ATCAACG79950.035.2530753
TCAACGC79900.035.2120934
CAACGCA81050.034.5700045
AACGCAG83450.033.779896
ACGCAGA89000.031.5963257
CGCAGAG90800.030.8566598
AGAGTAC93500.029.9277211
CAGAGTA94550.029.6328410
GCAGAGT95200.029.4305119
TCGCGTG658.9661696E-426.37967312
GAGTACT54650.026.16728412
AGTACGG53350.025.97243313
AGTACTT55000.025.87921713
GAGTACG53900.025.57702412
GTACTTT58250.024.43630814
Produced by FastQC (version 0.11.5)