FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702120
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8720.12419529425169487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.11351335953967982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3850.050.037361
GGTATCA13600.043.000861
TATACGT1800.042.62739670
GTATCAA35500.040.6024971
TGGTATC4700.039.5100942
TATCAAC40050.035.8910262
ATCAACG42450.033.592463
TCAACGC42500.033.3650744
CAACGCA43100.032.9732135
AACGCAG44050.032.26216
CGCAGAG47600.029.783888
AGAGTAC48550.029.27179111
ACGCAGA48600.029.2416767
GCAGAGT49600.028.5137089
CAGAGTA50250.028.34981310
CGGGCTA854.583426E-628.28354817
TGCGAAA1653.6379788E-1227.0456612
GTGCGAA1653.6379788E-1227.0456611
AGTACGG32300.026.78348713
ATTACGA1705.456968E-1226.62566447
Produced by FastQC (version 0.11.5)