FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837487
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80610.4386969812575545No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT56680.30846476736978273No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG55400.3014987316917072No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54500.2966007378555603No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53680.29213812124929317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53180.28941701356254496No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50500.27483187636157425No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG47480.25839638593361475No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG46270.251811305331684No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG45310.2465867785731273No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC45010.24495411396107838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT42990.23396083890661537No Hit
GTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTAATCTATTTGGGTT42050.22884515645552866No Hit
GTTCAGTGGCGGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG41420.22541656077022584No Hit
GGCCAAAAGTGTACGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA41080.22356620754323703No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT40910.22264103092974266No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACCGCCA40610.2210083663176937No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40290.2192668573981748No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA38000.20680418419286775No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA37860.20604227404057823No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35080.19091291530225793No Hit
AGCCAGGGCAGTCTCCACAACTCCTAATCTATTTGGGTTCTAATCGGGCC34170.1859604993123761No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC34080.18547069992876142No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33860.18427341254659216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32690.17790602055960122No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31590.17191958364875506No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCGTACACTTTTGGCCAGGGGA29250.1591847996747732No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT28310.1540691172236865No Hit
CCACTGAACCTGGCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATTAGGAGTTGTGGAGACTGCCCTGG28140.15314394061019207No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27670.1505860993846487No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC27620.1503139886159739No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACCGCCA27000.14693981508440604No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT26760.14563368339476687No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26610.1448173510887424No Hit
CTCCACAACTCCTAATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT26480.14410986309018786No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26070.14187855478705427No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25980.1413887554034396No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTCCACT25970.14133433324970462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25820.14051800094368014No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25660.1396472464839207No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25620.13942955786898084No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG25210.13719824956584728No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGA25180.13703498310464238No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25080.13649076156729273No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24900.13551116280006334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24830.1351302077239186No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24760.13474925264777385No Hit
CTCCTGCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTAAT24470.13317101018945984No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTCCACTCTCC24390.13273563295958013No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24040.13083085757885632No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA24000.13061316896391648No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23690.12892608219813256No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA23690.12892608219813256No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23610.12849070496825282No Hit
GTGTAAAATCTGTGCCTGATCCACCGCCACTGAACCTGGCAGGGACCCCG22650.12326617820969618No Hit
GCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGC21960.11951104960198358No Hit
CTCCTAATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGCCAGGTT21810.1186947172959591No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21680.11798722929740456No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21050.11455863361210174No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGGGCTCAGCTTCAGGGAGA20880.11363345699860734No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCGTACACTTTTGGCCAGGGGA20840.11341576838366747No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20550.11183752592535348No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20540.11178310377161851No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT20160.10971506192968983No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20150.10966063977595487No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19810.10781028654896607No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTC19660.10699395424294159No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTAATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGC19300.10503475670848283No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC19150.10421842440245836No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAGTAGTTGAATCCATTACTATGC19130.10410958009498843No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19120.10405515794125347No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18950.10312998132775905No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18910.10291229271281918No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT18740.10198711609932479No Hit
GTCCCTGCCAGGTTCAGTGGCGGTGGATCAGGCACAGATTTTACACTGAA18690.10171500533064995No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18650.1014973167157101No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTAATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGCCAGGTT18500.10068098440968562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)