FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837487
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71510.38917282135873615No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT50800.2764645409736232No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG50170.27303594528832037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48400.2634032240772316No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45510.24767522164782663No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45440.24729426657168185No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42970.23385199459914546No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC41200.2242192733880566No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA40820.22215123154612795No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG40790.22198796508492305No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG39690.21600152817407686No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT39180.21322599833359365No Hit
GTTCAGTGGCGGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG39140.2130083097186538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38540.20974298049455586No Hit
GTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTAATCTATTTGGGTT37890.20620554050178314No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA37860.20604227404057823No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36130.19662724144442925No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACCGCCA34690.18879045130659428No Hit
GGCCAAAAGTGTACGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA34050.1853074334675565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33880.1843822568540621No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33830.18411014608538728No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32790.1784502420969509No Hit
AGCCAGGGCAGTCTCCACAACTCCTAATCTATTTGGGTTCTAATCGGGCC30760.16740254488875295No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30200.1643549042795949No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC28870.15711675783284454No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT28040.15259971907284242No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28030.15254529691910745No Hit
CTCCACAACTCCTAATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT27500.1496609227711543No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG27440.1493343898487445No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCGTACACTTTTGGCCAGGGGA27340.14879016831139485No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26960.14672212646946617No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26920.14650443785452633No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26470.14405544093645287No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG26410.1437289080140431No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26290.1430758421692235No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25720.1399737794063305No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC25330.13785131541066684No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT25270.13752478248825706No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTCCACTCTCC24850.13523905203138853No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24620.1339873424954843No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTCCACT23750.12925261512054234No Hit
CTCCTAATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGCCAGGTT23710.1290349265056025No Hit
CTCCTGCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTAAT23470.12772879481596333No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGA23120.12582401943523955No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACCGCCA22940.12484442066801017No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22720.12364713328584093No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22710.12359271113210597No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22490.12239542374993674No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22440.12212331298126192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22040.11994642683186331No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21930.1193477831407787No Hit
CCACTGAACCTGGCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATTAGGAGTTGTGGAGACTGCCCTGG21380.1163545646853556No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21290.1158647653017409No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21120.1149395886882465No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA21070.11466747791957167No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGGGCTCAGCTTCAGGGAGA20940.11395998992101712No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20900.11374230130607728No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20860.11352461269113741No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20800.11319807976872762No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT20180.10982390623715976No Hit
GTCCCTGCCAGGTTCAGTGGCGGTGGATCAGGCACAGATTTTACACTGAA20180.10982390623715976No Hit
GTGTAAAATCTGTGCCTGATCCACCGCCACTGAACCTGGCAGGGACCCCG19490.10606877762944716No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTATGATGACTCAGTCTC19450.10585108901450732No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19350.10530686747715766No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC19220.10459937947860311No Hit
GCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGC19020.10351093640390381No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCGTACACTTTTGGCCAGGGGA18980.10329324778896394No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATTCAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA18850.1025857597904094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)