FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441511
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42600.29552323915669043No Hit
GTACACAGTGGCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA38560.2674970915934738No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA37820.262363589317043No Hit
CCCTTGGACAGCCGGCCACCATCTCCTGCAGGTCTAGTGAAAGCCTCGTG31980.22185054432467044No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28060.19465685659006418No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26320.18258618907521343No Hit
GTGTATTACTGTGCGAGGGCGGGCAGTACAATTTTTGGAGAGGCTTTTGA25510.17696708523209326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24430.1694749467745997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24390.1691974601650629No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23150.16059537526942216No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAGTCTCCAAGGCGCCTAATTTATAAGGTTT22940.15913857056935396No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21490.14907968097364502No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21250.14741476131642423No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA21220.14720664635927164No Hit
GTCCTGTGCAGCCTCTGGATTCATGTTTAGTGACTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG20690.14352994878290903No Hit
ACTGTGTACCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACC20570.14269748895429865No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20050.13909016303032026No Hit
GTGTACCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGC19240.13347105918720012No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18670.1295168750013007No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC18240.12653389394878012No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17750.12313468298195435No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17190.11924987044843918No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17170.11911112714367077No Hit
CAAGGTACACAGTGGCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17160.11904175549128657No Hit
GTCTGGGACCCCAGAGTCCCGGTAAGAAACCTTATAAATTAGGCGCCTTGGAGACTGGCCTGGCCTCTGGTGAAA16960.11765432244360259No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTAATTTATAAGGTTTCTTACCGGGAC16930.11744620748644999No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.11633626104830279No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16750.1161975177435344No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16690.1157812878292292No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16540.1147407130434662No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16030.11120275877187201No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15870.11009281233372481No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15810.10967658241941962No Hit
CCCTGAGACTGTCCTGTGCAGCCTCTGGATTCATGTTTAGTGACTATTGG15490.10745668954312522No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15450.10717920293358844No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14880.10322501874768905No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG14730.10218444396192605No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT14460.10031140934755266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5500.048.1822781
TGGTATC6050.044.3709342
GGTATCA18000.042.2550931
GTATCAA45250.040.3103561
CTAGCGC2600.037.09289628
TATCAAC50850.035.6003382
ACTAGCG2900.034.43861827
TAGCGCT2900.033.26262729
ATCAACG54650.033.2508773
TCAACGC55350.032.7681774
CAACGCA56250.032.3050735
AACGCAG56800.032.113446
CGAGACT3450.031.93197623
ACGAGAC3350.031.85529922
CGTATGC1850.031.82196241
TCGTATG1750.031.64273640
GAGACTA3300.031.29696724
CTTATAC3700.030.6952761
TATACAC3600.030.591923
AGACTAG3400.030.37752325
Produced by FastQC (version 0.11.5)