FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441511
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39890.2767235213605724No Hit
GTACACAGTGGCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA36420.2526515579832551No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA34330.23815288263495735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32310.22413980885334903No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25570.17738331514639846No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23970.16628385076492652No Hit
GTGTACCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGC23450.16267652484094813No Hit
GTGTATTACTGTGCGAGGGCGGGCAGTACAATTTTTGGAGAGGCTTTTGA22430.1556006162977598No Hit
ACTGTGTACCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACC22270.15449066985961257No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22040.152895121854776No Hit
CCCTTGGACAGCCGGCCACCATCTCCTGCAGGTCTAGTGAAAGCCTCGTG21810.15129957384993942No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21570.1496346541927186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21270.14755350462119263No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19740.13693964180641008No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19150.1328467143157423No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAGTCTCCAAGGCGCCTAATTTATAAGGTTT18370.12743572542977472No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC18260.12667263725354852No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17830.12368965620102795No Hit
GTCCTGTGCAGCCTCTGGATTCATGTTTAGTGACTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17810.12355091289625955No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17590.12202473654380716No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA17470.12119227671519676No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16810.11661374765783959No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16370.11356139495293481No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16360.1134920233005506No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15990.11092527216233522No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15820.1097459540718038No Hit
GTCTGGGACCCCAGAGTCCCGGTAAGAAACCTTATAAATTAGGCGCCTTGGAGACTGGCCTGGCCTCTGGTGAAA15740.10919098085273023No Hit
CAAGGTACACAGTGGCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15530.10773417615266204No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15490.10745668954312522No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15000.10405747857629945No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14520.10072763926185788No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14500.10058889595708946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)