FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781199
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30510.17128911480412912No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29680.16662933226439045No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28300.15888174201759603No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTACTTCTGGGGCTGGATCCGGCA24770.13906363073412908No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACT23540.13215816986198622No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21460.12048064253348448No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20630.11582085999374578No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19880.11161021312048794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.11015052220442521No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19290.10829783758019176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19230.10796098583033115No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT18890.10605215924778758No Hit
ACTATAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTCGACATGTCC18630.10459246833172486No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18550.10414333266524403No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18210.10223450608270047No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18100.1016169445412893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)