FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781199
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30910.1735347931365333No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27440.154053533602927No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26130.14669893706430331No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25260.1418145866913242No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACT23830.13378628665297926No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23720.1331687251115681No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTACTTCTGGGGCTGGATCCGGCA21120.1185718159509409No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT19470.10930839282977366No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18750.10526617183144611No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18440.10352577112383288No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18360.10307663545735204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA554.675063E-448.88632270
GTATCAA64850.041.0904351
TATCAAC72650.036.463582
ATCAACG76850.034.398913
TCAACGC77150.034.1917574
GTGGTAT10900.033.796141
CAACGCA78650.033.69925
AACGCAG79950.033.4042056
CGCAGAG86050.031.1168
CTAGTCG450.00473823930.5131849
AGAGTAC87600.030.40868611
ACGCAGA89000.029.9689187
GCAGAGT90150.029.548549
GGTATCA39850.028.1643071
AGTACGG61800.027.71893313
GAGTACG62450.027.59378612
CAGAGTA108600.024.5917710
GTACGGG70350.024.109514
GAGTACT39200.024.08167512
AGCGAAT1400.001553090624.00667870
Produced by FastQC (version 0.11.5)