FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556092
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.10070276141357906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3550.041.6433331
GTATCAA22600.038.9436761
TGGTATC4150.034.7941742
TATCAAC26050.034.5779232
GGTATCA9150.034.1920931
ATCAACG26650.033.670433
TCAACGC26800.033.4819764
CAACGCA27550.032.5704885
GACGGAC1950.031.73249226
ACGGACT1850.031.59239627
AACGCAG28400.031.4746136
ACGCAGA30900.028.9073147
GCCTAAC605.5302837E-428.6499671
ACGAGAC2050.028.49738922
CGAGACG2200.028.11893823
CGCAGAG31900.028.1088248
TTTCGAC500.00736928327.8726647
CGTATTC500.0075147527.76127241
CCCACGA2000.027.48912819
AGAGTAC32800.027.0233211
Produced by FastQC (version 0.11.5)