FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323875
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25500.1926163723916533No Hit
GTATAATAACTGGCCTCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16060.12131054669058634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15960.12055518836748183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.043.7252851
GTATCAA54250.040.8809661
GTGGTAT7750.039.547891
TATCAAC59800.036.7218442
ATCAACG63850.034.2653243
TCAACGC63950.034.1897664
CAACGCA65500.033.4192855
AACGCAG67150.032.546946
TAGGTCG450.00471337530.545455
ACGCAGA71900.030.4923447
CGCAGAG73100.030.1328128
AGAGTAC73950.029.83180411
GCAGAGT75400.029.3047929
CAGAGTA76300.029.04920410
CGATTAT500.007498692727.77626245
AGTACGG47900.026.330713
GAGTACG48500.026.00201212
AGTACTT37500.024.6520413
GAGTACT38300.024.58297312
TGGTATC16050.023.8041652
Produced by FastQC (version 0.11.5)