FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657200
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGC42320.6439440048691418No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29270.44537431527693244No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGGGTGTTGTCGTAGGA26670.4058125380401704No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26110.39729153986609855No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTG25790.39242239805234325No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24400.37127206329884355No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22080.3359707851491175No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21900.3332318928788801No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19850.30203895313451No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19530.2971698113207547No Hit
GGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGC18920.2878880097382836No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCAC18820.2863664029214851No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGG18730.2849969567863664No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18310.27860620815581255No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17430.2652160681679854No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAG17300.2632379793061473No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT17220.26202069385270843No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16840.256238587948874No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16570.2521302495435179No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC16400.2495435179549604No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16210.24665246500304322No Hit
GAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT16190.2463481436396835No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCAC15920.24223980523432742No Hit
ACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAG15860.24132684114424835No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15330.23326232501521604No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14920.22702373706634205No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14450.21987218502738892No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13890.2113511868533171No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13890.2113511868533171No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13540.20602556299452224No Hit
GTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGG13290.20222154595252584No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT12990.19765672550213026No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAC12640.19233110164333536No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12560.19111381618989653No Hit
CCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG12370.18822276323797932No Hit
GATTATTACTGCCAGTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGG12350.1879184418746196No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12350.1879184418746196No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG12150.1848752282410225No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12050.18335362142422398No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11790.17939744370054778No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGG11610.1766585514303104No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11570.176049908703591No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAG11170.16996348143639683No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11170.16996348143639683No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11160.16981132075471697No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11140.16950699939135727No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11090.168746195982958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10970.16692026780279975No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCA10740.1634205721241631No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTA10590.1611381618989653No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10500.15976871576384663No Hit
GTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGG10460.1591600730371272No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGC10280.15642118076688985No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10270.15626902008521No Hit
GTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCC10260.15611685940353012No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10190.15505173463177113No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10170.15474741326841143No Hit
ATCTATGGGAACGCCAATCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC10150.15444309190505173No Hit
CCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG10040.15276932440657334No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT9990.15200852099817408No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9880.1503347534996957No Hit
CAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGC9870.15018259281801583No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9850.14987827145465613No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT9780.14881314668289713No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTC9720.147900182592818No Hit
GTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG9700.1475958612294583No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9640.14668289713937918No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC9440.1436396835057821No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9400.1430310407790627No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9360.14242239805234327No Hit
ACACTACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATC9070.1380097382836275No Hit
ACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCT9000.13694461351186854No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8990.13679245283018868No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8770.1334449178332319No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACA8540.12994522215459525No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8470.12888009738283626No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC8300.12629336579427877No Hit
GGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGAC8220.12507608034083995No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG8170.12431527693244067No Hit
CATCGGGGCAGGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAA8120.12355447352404138No Hit
ACACCACACTACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCC8080.12294583079732198No Hit
GTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGCC8050.12248934875228241No Hit
GGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG8040.12233718807060255No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8030.1221850273889227No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8020.12203286670724285No Hit
CATCTATGGGAACGCCAATCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC7940.12081558125380401No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG7930.12066342057212417No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC7930.12066342057212417No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC7930.12066342057212417No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7920.12051125989044431No Hit
GGGTCACCATCTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAACTTC7900.12020693852708461No Hit
GTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTA7880.11990261716372488No Hit
CTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCGCC7860.11959829580036518No Hit
GCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT7660.11655508216676812No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7660.11655508216676812No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7650.11640292148508825No Hit
CCACACTACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGG7620.11594643944004869No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA7580.11533779671332929No Hit
ATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGCCCCT7580.11533779671332929No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTACGACAACA7550.11488131466828973No Hit
GTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCG7490.11396835057821059No Hit
ACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGG7400.1125989044430919No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7370.11214242239805233No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7350.11183810103469263No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT7170.10909920876445527No Hit
CCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCGCCAACATC7150.10879488740109557No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC7150.10879488740109557No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC7080.10772976262933658No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTC7050.10727328058429701No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACAC7020.10681679853925745No Hit
AGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGG6930.10544735240413877No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT6830.10392574558734022No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC6780.10316494217894095No Hit
CATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGCCCCTCAGGGGTC6740.10255629945222156No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT6730.10240413877054169No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG6710.10209981740718199No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT6670.10149117468046256No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG6650.10118685331710285No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTACGACAACA6600.10042604990870359No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6580.10012172854534389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2500.057.8816761
TGGTATC2850.048.3224562
ACGGGAT507.102426E-848.15762716
GGTATCA9850.046.5208051
GTATCAA27100.043.225641
CGTTTAG908.122053E-539.71057570
TATCAAC30050.038.6117322
ATCAACG32200.035.8034063
TCAACGC32350.035.4138454
CAACGCA33050.034.650595
AACGCAG33700.033.8776256
ACGCAGA35200.032.5316737
CGCAGAG35300.032.34218
AGAGTAC35100.032.3279811
GCAGAGT35600.032.0695539
CAGAGTA35350.032.00451710
TCGCTTT500.007251347427.96568561
TGCGTAT500.007711588427.6153436
GAGTACT14850.027.55448312
TGTTATA500.00780982827.5438022
Produced by FastQC (version 0.11.5)