FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561386
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69100.4425555243866668No Hit
GTTTTATACATCTCCAACAATAAGCACTGCTTAGCTTGGTACCAACAGAA58920.3773570404755775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46930.30056629174336136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45600.29204821869800296No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGCAGTGCTTATTGTTGGAGATGTATAAAACACT44210.28314587168067346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42760.2738592506913729No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42160.27001651097166235No Hit
ATATCATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCAT40970.2623950771942364No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40410.25880852012250655No Hit
CTCCTAAGGTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT39890.2554781456987574No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35500.22736210008287508No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34620.22172608182729958No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA31700.2030247485247082No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA31360.20084719601687218No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC31200.19982246542494939No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31130.19937414579098314No Hit
GCTTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGGTACTCATT30490.19527522342329187No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29870.19130439237959096No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC29580.18944706818173085No Hit
GTTTATTACTGTCAGCAATATCATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAA29310.1877178353078611No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28500.18253013668625184No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27480.17599747916274386No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG27480.17599747916274386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26840.1718985567950526No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG26620.17048955223115872No Hit
CAATAAGCACTGCTTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTAC26400.16908054766726485No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACATCTCCAA25650.16427712301762665No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGTCTCAGGGAGA25400.16267598146774723No Hit
AACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTACCCGGGAA24800.15883324174803667No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC24040.1539657714364033No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23620.1512758536326059No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23560.15089157966063485No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATCATAGTCCT23130.14813761619484228No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22680.14525556140505935No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22290.14275778058724747No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21920.14038809109342595No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21730.1391712235155176No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21500.13769817328962858No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21500.13769817328962858No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21430.13724985365566233No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC21300.13641726004972504No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA20970.13430375320388424No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20970.13430375320388424No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20930.13404757055590352No Hit
GGCCAAAAGTGTACGGAGGACTATGATATTGCTGACAGTAATAAACTGCC20920.13398352489390836No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20850.13353520525994214No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACATCTCCAACAATAAGCACTG20790.13315093128797106No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20640.13219024635804344No Hit
TCATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT20600.13193406371006272No Hit
CTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20460.13103742444213026No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTACCTTAGGAGGCTGTCCTGGTTTCTGTTGGTA20300.13001269385020744No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19760.12655422810246794No Hit
GTAAATGAGTACCTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTA19570.1253373605245596No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA18760.12014966190295033No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC18440.11810020071910471No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA18130.11611478519725424No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18070.11573051122528318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17620.11284845643550025No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17590.11265631944951474No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17480.11195181716756779No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG17270.11060685826566909No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16950.10855739708182346No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16870.10804503178586204No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16650.10663602722196819No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16630.10650793589797783No Hit
GTGTCTGGTGACTCCATCAGCAGGGGTGGTTATTATTGGAACTGGATCCG16550.10599557060201642No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16490.10561129663004536No Hit
CTGCTTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTACCCGGGAATC16450.10535511398206467No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16440.10529106832006949No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16410.10509893133408393No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC16410.10509893133408393No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG16290.10433038339014183No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16160.10349778978420454No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG15920.10196069389632031No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15900.10183260257232997No Hit
GTATATTACTGTGCGAGGCAATGGGAATCAGTACGGGGGCCGAACTGGTT15850.1015123742623541No Hit
GTCCTGGTTTCTGTTGGTACCAAGCTAAGCAGTGCTTATTGTTGGAGATG15770.10100000896639268No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15730.10074382631841197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002337196669.13737539
GTGGTAT6650.049.5631641
TGGTATC7000.047.5754782
GTATCAA55650.043.6794851
GGTATCA20100.041.8484571
TATCAAC63100.038.359172
CTTATAC3800.036.1398051
ATCAACG68300.035.5895043
TCAACGC68900.035.3294144
CGTATGC2900.034.62676241
CAACGCA71000.034.2844625
AACGCAG71750.033.9260836
AATCTCG2600.033.17064736
CCGAAAC702.0024527E-532.54305368
GCCACGT800.003596076532.2802170
ATCTCGT2500.031.75505337
CGCAGAG77550.031.3786858
AGAGTAC78150.031.09385711
TCGTATG2900.031.01962740
GCAGAGT79100.030.8505889
Produced by FastQC (version 0.11.5)