FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561386
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67570.4327565381014048No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46860.3001179721093951No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44470.28481105889254804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42080.2695041456757009No Hit
GTTTTATACATCTCCAACAATAAGCACTGCTTAGCTTGGTACCAACAGAA40810.26137034660231356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40120.25695119592464644No Hit
ATATCATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCAT39040.25003426442916743No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36800.23568803614224795No Hit
CTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC35490.2272980544208799No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35180.22531263889902947No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG33930.21730693114963245No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32810.21013381700617273No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA31660.20276856587672748No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31420.20123146998884325No Hit
CTCCTAAGGTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT30270.193866218859398No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA28740.18406723257413604No Hit
GGCCAAAAGTGTACGGAGGACTATGATATTGCTGACAGTAATAAACTGCC28620.18329868463019394No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28250.18092899513637242No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG28230.18080090381238206No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC27180.17407610930288858No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGCAGTGCTTATTGTTGGAGATGTATAAAACACT27120.17369183533091753No Hit
GTTTATTACTGTCAGCAATATCATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAA26810.17170641980906706No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26540.1699771869351973No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26410.16914459332926002No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26200.16779963442736132No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24870.15928156138200292No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23870.1528769951824853No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23200.14858593582880852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23090.14788143354686156No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC23030.1474971595748905No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA22850.14634433765897736No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22820.1461522006729918No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGTCTCAGGGAGA22390.14339823720719924No Hit
GGACTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22110.14160495867133432No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT21790.1395554974874887No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATCATAGTCCT21690.13891504086753692No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20960.13423970754188908No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC20680.13244642900602413No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20670.13238238334402896No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20620.13206215503405308No Hit
GCTTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGGTACTCATT20180.12924414590626532No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20020.12821941531434253No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19990.12802727832835697No Hit
TCATAGTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT19960.12783514134237145No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19670.12597781714451137No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC19610.1255935431725403No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19530.1250811778765789No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19430.12444072125662714No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19100.12232721441078631No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18900.12104630117088279No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC18250.11688333314119634No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17980.1151541002673266No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17740.11361700437944237No Hit
CAATAAGCACTGCTTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTAC17670.11316868474547613No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACATCTCCAA17410.11150349753360154No Hit
AACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTACCCGGGAA17330.11099113223764015No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTACCTTAGGAGGCTGTCCTGGTTTCTGTTGGTA17280.11067090392766427No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17160.10990235598372215No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG16860.10798098612386686No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16480.10554725096805018No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16260.1041382464041563No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16060.10285733316425277No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16010.10253710485427689No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15820.10132023727636855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7000.053.5060961
TGGTATC7600.049.7005352
GGTATCA20850.040.5418781
GTATCAA51950.040.2815861
TATCAAC59050.035.472512
ATCAACG63950.032.82823
TCAACGC64550.032.4470334
TCTATAC851.2711098E-732.285483
CAACGCA66150.031.7546715
AACGCAG67200.031.3084936
CGCAGAG72600.028.9325628
GCAGAGT75050.027.9880629
AGAGTAC75100.027.87728311
CAGAGTA75350.027.83026310
ACGCAGA75650.027.8113787
GTACGGT659.001089E-426.36266314
CTAGACT1058.129555E-726.1174454
TGTTAGA14600.025.37837670
AAGTGAA13500.024.50557570
TGGACCG851.511523E-424.1885195
Produced by FastQC (version 0.11.5)