FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649458
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24820.38216482051187295No Hit
TTATTACTGCATGCAAGCTCTTCACTCTCCTTGGACTTTCGGCGGGGGGA16630.25605966821565057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16520.25436594822144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15970.24589734825038725No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15680.24143208644746852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15060.23188566466191807No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14640.22541873377493232No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14090.21695013380387956No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12970.1997049847719175No Hit
GTGCAGCCACAGTTCGTTTGAGATCCACCTTGGTCCCCCCGCCGAAAGTC12550.19323805388493173No Hit
GTCCCTGACAGGTTCAGTGGCAGTGTATCAGGCACAGATTTTACACTGAA11820.18199791210517077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11750.18092009029067316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11550.1778405993921085No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG11530.17753265030225204No Hit
GGTCTAGTCAGAGCCTCCTGCATCATAATGGATACTACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC11250.17322136304426153No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT11180.17214354122976389No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATACACTGCCA11170.17198956668483567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11020.16967994851091217No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10740.16536866125292168No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCGCAGCTTCAGGGAGA10240.15766993400651005No Hit
GTTCAGTGGCAGTGTATCAGGCACAGATTTTACACTGAAAATCAGTAGAG10140.1561301885572277No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC9590.14766158858617492No Hit
CTACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGC9500.14627581768182085No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9430.1451979958673232No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTATTGGACTTGGATCCGCCAGTC9340.14381222496296914No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9220.14196453042383034No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9190.14150260678904564No Hit
GTCCAAGGAGAGTGAAGAGCTTGCATGCAGTAATAAAGTCCAACATCCTC9140.14073273406440448No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9000.13857709043540922No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGATCCACCTTGGTCC8910.13719131953105512No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8890.13688337044119867No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT8810.1356515740817728No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG8800.13549759953684457No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8790.13534362499191635No Hit
GTATCAGGCACAGATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGA8780.1351896504469881No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG8730.13441977772234695No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8660.1333419559078493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8500.1308783631889976No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8390.12918464319478704No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATACACTGCCA8150.12548925411650946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8050.12394950866722713No Hit
CTCCTTGGACTTTCGGCGGGGGGACCAAGGTGGATCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC8030.12364155957737066No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7830.12056206867880602No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAAGCTCTTCACTCT7750.11933027231938015No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7740.11917629777445193No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7610.11717462869038489No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATGTTGTGCTGACTCAGTTTCCACTCTCCCTGCCCGTCACCC7600.11702065414545668No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7390.1137871887019638No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATGTTGTGCTGA7360.11332526506717909No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7340.11301731597732263No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7160.11024577416861445No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATGTTGTGCTGACTCAGTTTCCACT7030.10824410508454743No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC7020.10809013053961919No Hit
GAGTGAAGAGCTTGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT6990.1076282069048345No Hit
GTCTATTACTGTGCGAGAGCGCGATTTGTCGAGTGGTTGGGCCCCTCCTACTGTGACTACTGGGGCCAGGGAACC6910.10639641054540863No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6910.10639641054540863No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA6890.10608846145555217No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATGTTGTGCTGACTCAGTTTCCACTCTCC6800.10470269055119806No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA6740.10377884328162867No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6680.1028549960120593No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6630.10208512328741812No Hit
GGATACTACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAAT6560.1010073014729205No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTGGTTACTATTGGACTTGGATCCGCCAGTCCCCAGGGAAGGGA6550.10085332692799226No Hit
CTTTATTACTGCATGCAAGCTCTTCACTCTCCTTGGACTTTCGGCGGGGGGACCAAGGTGGATCTCAAACGAA6520.10039140329320757No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6510.10023742874827933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)