FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006831
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21450.2130446917109227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6800.045.4580651
GTATCAA51050.042.1168561
GGTATCA19600.036.9746171
TGGTATC8400.036.782912
TATCAAC59500.036.119372
ATCAACG63050.034.0126233
TCAACGC63050.033.9362644
CAACGCA64350.033.2358175
AACGCAG65500.032.9122926
ACGCAGA70100.030.7036787
CGCAGAG71300.030.331138
AGAGTAC73250.029.7093611
GCAGAGT73650.029.5029379
CAGAGTA73900.029.4495110
GAGTACG43800.025.97712912
AGTACGG43600.025.70710613
TACGGGG18150.025.12818515
GAGTACT41850.024.40326912
AGTACTT41550.024.3356513
ATAGCGG1901.8189894E-1124.20601863
Produced by FastQC (version 0.11.5)