FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189384
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68490.5758443025969746No Hit
ACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC53040.4459451278981389No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44960.37801080223039824No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44270.3722094798652075No Hit
GAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT43390.3648106919211962No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43030.36178391503500973No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42640.35850490674164104No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41010.3448003336180746No Hit
CCTCAAGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAG37890.3185682672711252No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37400.3144484876204825No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34940.2937655122315417No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC34880.2932610494171773No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT33660.28300363885843427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32670.2746800024214215No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG30700.25811680668312337No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAA30630.25752826673303153No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30310.2548377983897547No Hit
GTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCA28610.2405446853160964No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAC28360.23844275692291136No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27750.23331405164353983No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27460.23087581470744523No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25990.21851647575551714No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT25290.212631076254599No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC24500.2059889825321343No Hit
ATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT24110.20270997423876563No Hit
AGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT23210.19514303202329944No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22830.1919481008656582No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22630.19026655815111015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22520.18934170965810873No Hit
CCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAG22420.18850093830083473No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC22000.18496969860028384No Hit
ACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA21530.18101807322109595No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21110.1774868335205451No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20910.17580529080599705No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20900.17572121367026963No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20880.17555305939881485No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTAC20830.17513267372017785No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTA20720.17420782522717643No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20390.17143327974817216No Hit
ACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA20210.16991989130507892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAAGCG20190.16975173703362412No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCA20120.16916319708353234No Hit
GTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGA20030.1684065028619857No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT19950.1677338857761665No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19760.16613642019734587No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19580.1646230317542526No Hit
GTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCA19350.1626892576325224No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19300.16226887195388537No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18130.15243184707377938No Hit
GAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATC17720.1489846845089559No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17380.14612606189422422No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17350.14587383048704203No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA17190.1445285963154036No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16820.14141774229348975No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTT16660.1400725081218513No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16350.13746611691430186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.13645719128557304No Hit
GTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTC16220.13637311414984563No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15890.13359856867084136No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACC15890.13359856867084136No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15640.13149664027765634No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15630.13141256314192892No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15600.13116033173474673No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGC15400.1294787890201987No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15160.12746093776274106No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAG15130.12720870635555884No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGT15100.12695647494837664No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACT14990.12603162645537522No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14940.12561124077673821No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14690.12350931238355317No Hit
GAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAAC14560.12241630961909694No Hit
CTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTC14540.12224815534764215No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14290.1201462269544571No Hit
GAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGACCCCA14040.11804429856127206No Hit
GCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTT13930.11711945006827064No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13790.115942370168087No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG13740.11552198448944999No Hit
CTGCAAAGTGGATGCAGCAAAGATCAGGAGCTTAGGGGCTCTCCCTGGTT13560.11400859604635677No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13540.11384044177490196No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13500.11350413323199236No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13450.11308374755335535No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13250.11140220483880732No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCA13240.11131812770307992No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13140.1104773563458059No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAG13130.1103932792100785No Hit
CCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAG12760.10728242518816464No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTG12690.10669388523807281No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG12620.106105345287981No Hit
GTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAA12600.10593719101652621No Hit
TTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTG12580.1057690367450714No Hit
GTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCA12500.10509641965925219No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC12480.10492826538779738No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12440.10459195684488778No Hit
CCCCTAAGCTCCTGATCTTTGCTGCATCCACTTTGCAGAGTGGGGTCCCA12290.10333079980897675No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCCTCAA12260.10307856840179454No Hit
GCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTG12250.10299449126606715No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12070.10148110282297391No Hit
ACCTTGATGGGACCCCACTCTGCAAAGTGGATGCAGCAAAGATCAGGAGCTTAGGGGCTCTCCCTGGTTTCTG11920.10021994578706288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3550.051.1178861
ATCGTAT306.224554E-446.12969639
GTATCAA39200.041.8383751
ATCGCGT350.001336541339.49609837
CTTATAC3600.037.0926171
TATCAAC44950.036.332542
ACTAGCG3450.035.82443227
TAGCGCT3600.035.30624829
CTAGCGC3600.035.29582628
ATCAACG46850.035.078323
TCAACGC47250.034.7813574
CAACGCA48050.034.4160355
TATACAC4150.033.8253943
AACGCAG49100.033.819516
TCGTATG2000.032.8939740
ACGAGAC3850.032.0483522
AGCGCTC4200.031.09352330
CACGAGA4000.030.8465421
CGCAGAG54050.030.7119268
ATCTCCG4150.030.51508910
Produced by FastQC (version 0.11.5)