FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189384
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62840.5283407209109926No Hit
ACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC51340.4316520148244806No Hit
GAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT42870.3604386808633713No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42200.35480551276963535No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40160.3376537770812454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39140.32907790923705044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37730.3172230330994868No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36720.30873124239101923No Hit
CCTCAAGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAG34910.2935132808243595No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33720.28350810167279866No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC32810.27585708232160516No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32600.2740914624713297No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT32520.2734188453855105No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31390.2639181290483141No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG29970.25197917577502305No Hit
GTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCA28370.23852683405863875No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28190.23701344561554552No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAA28130.23650898280118113No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25720.21624639309087731No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25530.21464892751205666No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAC25520.21456485037632927No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24730.20792275665386453No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC23360.1964041890592105No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22920.1927047950872048No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22720.19102325237265677No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTA21820.1834563101571906No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21720.1826155387999166No Hit
AGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT21670.18219515312127957No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21590.18152253603546037No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCA21420.18009322472809453No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21340.17942060764227533No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21070.17715052497763548No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20990.17647790789181628No Hit
ACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA20960.17622567648463405No Hit
ACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA20850.17530082799163263No Hit
CCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAG20390.17143327974817216No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAAGCG20380.17134920261244477No Hit
ATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT20290.17059250839089815No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19550.1643708003470704No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTAC19440.163445951854069No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18200.15302038702387116No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACT18190.15293630988814377No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18160.15268407848096158No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18000.15133884430932315No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17930.1507503043592313No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17900.15049807295204912No Hit
GTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGA17820.14982545586622992No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAG17720.1489846845089559No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17600.14797575888022707No Hit
GTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCA17580.14780760460877226No Hit
GAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATC17530.14738721893013526No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17150.14419228777249402No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16770.14099735661485271No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16390.13780242545721147No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTT16030.134775648571025No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGC15850.13326226012793177No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15800.13284187444929477No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15490.1302354832417453No Hit
GAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15370.1292265576130165No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15010.12619978072683002No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACC14630.12300484956918875No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGT14510.12199592394045992No Hit
GTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTC14410.12115515258318592No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA14190.11930545559718309No Hit
CTGCAAAGTGGATGCAGCAAAGATCAGGAGCTTAGGGGCTCTCCCTGGTT14190.11930545559718309No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14100.11854876137563645No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCA13810.11611052443954181No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC13670.11493344453935819No Hit
GAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAAC13480.11333597896053754No Hit
GTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13350.11224297619608134No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13340.11215889906035392No Hit
GTACTAATACTCTGACTTGCCCGGCAAGTGATGGTGACT13140.1104773563458059No Hit
GTCTCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAAGT13050.10972066212425928No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC12990.10921619930989487No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12950.10887989076698526No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG12790.10753465659534683No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12790.10753465659534683No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12780.10745057945961943No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12580.1057690367450714No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12510.10518049679497958No Hit
CTCCTGATCTTTGCTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGA12460.10476011111634256No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAG12230.10282633699461234No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12230.10282633699461234No Hit
CCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAG12100.10173333423015611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)