FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729344
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.12723762723762724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32000.040.77991
GGTATCA13650.038.743661
GTGGTAT3400.037.371051
TATCAAC37650.034.7538032
ATCAACG39050.033.859623
TCAACGC39350.033.51424
CAACGCA40050.033.0141835
GCGTAGT1056.657501E-1032.74386629
AACGCAG41650.031.7459346
CGAGACG1305.456968E-1231.69283523
GACGCGT1101.1077645E-931.22763326
TCGTATG1001.3773388E-831.21086740
CGCGTAG1151.7716957E-929.88631228
ACGCAGA44600.029.7088817
CGCAGAG45100.029.5317388
CTCGTAT953.1005038E-729.15871839
TGGTATC4600.029.1171672
AGAGTAC45300.029.02048311
CAGAGTA46300.028.46782710
GCAGAGT47050.028.2328959
Produced by FastQC (version 0.11.5)