FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729344
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12740.1746775184275184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4800.042.163861
GTATCAA38400.040.645251
GGTATCA15250.039.3638731
TATCAAC42400.036.626332
TCAACGC44650.034.965364
ATCAACG45150.034.454853
CAACGCA45650.034.3305745
TCGCGAC400.002688654734.2533624
TGGTATC5650.033.978222
AACGCAG46950.033.4528736
ACGCAGA50050.031.3808677
CGCAGAG50850.031.0217518
AGAGTAC51950.030.1672911
CAGAGTA52250.030.12505710
GCAGAGT53050.029.735279
AGTACGG30950.026.53789313
GAGTACG31300.026.3468712
GAGTACT29850.024.53159512
AGTACTT29450.024.40337413
CGGGACT1052.4182309E-522.824614
Produced by FastQC (version 0.11.5)