FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565536
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCTCGGCTCTCAGGTTGTTTATTTGCAGATACA35350.2258012591214766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32970.2105987981113178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29490.1883699895754553No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27270.17418954275085338No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21240.13567238313267788No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21150.13509750015330213No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGGTTGTTTATTTGCAGATA20840.13311734766878563No Hit
ATGTATTACTGTGCGAGAGAGGCGTGGTTATTATCCTACTATTTTGACAA20080.12826278028739038No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19850.12679363489565235No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18860.12046992212251906No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG18520.11829814197821065No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18300.11689287247306994No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17960.11472109232876153No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17650.11274093984424505No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT17010.10865288310201746No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16900.1079502483494471No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16520.10552296465874947No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16080.102712425648468No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTCGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT15920.1016904114629111No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15860.10130715614332726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.041.4060131
TATCAAC59350.038.029562
GTATCAA64450.036.05341
ATCAACG62850.035.7689133
TCAACGC63550.035.2462924
GTGGTAT9400.035.208911
TGGTATC9450.034.6411672
CAACGCA64900.034.4998975
AACGCAG66100.033.7673536
CGCAGAG71350.031.330928
AGAGTAC72200.030.86679511
GCAGAGT73800.030.2907989
ACGCAGA74050.030.1885367
TGTCGTT1104.1683903E-430.06947970
CAGAGTA75200.029.58967410
AGTACGG53850.026.63457313
GAGTACG54350.026.51272612
GAGTACT31300.025.3809312
AGTACTT31050.025.25623513
TACGGGA23750.023.3258815
Produced by FastQC (version 0.11.5)