FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286379
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.12818928169691826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54350.039.385291
GGTATCA23000.038.9904481
TATCAAC63800.033.8733862
GTGGTAT8700.033.5695271
TCAACGC66550.032.473664
ATCAACG67300.032.2138753
CAACGCA68250.031.614455
TGGTATC9500.031.464752
AACGCAG71300.030.2620816
CTCGTAT2650.028.74947539
ACGCAGA76100.028.300457
CGCAGAG77150.027.9142028
AGAGTAC78200.027.49547211
CAGAGTA80300.026.77641310
TCGTATG3000.026.5705340
GCAGAGT81750.026.469549
CGCATAA1701.2622264E-524.82485670
ATCTCCG4150.023.17413510
AGTACGG49950.023.10454413
GAGTACG50050.023.0583812
Produced by FastQC (version 0.11.5)