FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286379
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34760.2702158539590587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62600.040.2616771
GGTATCA25850.039.0532231
GTGGTAT9000.036.245261
TATCAAC70750.035.310542
TCAACGC73200.033.9793474
ATCAACG73400.033.959853
CAACGCA74950.033.2644545
AACGCAG76350.032.7877856
TGGTATC9800.032.5653882
CGCAGAG81650.030.5756078
ACGCAGA82350.030.3572927
AGAGTAC84500.029.74699611
CAGAGTA86550.029.0028510
GCAGAGT87400.028.6424229
AGTACGG47150.026.43974113
GAGTACG48050.025.94249712
TACGGGG21550.025.59178415
GAGTACT51750.024.28619412
AGTACTT52750.023.7627213
GTACGGG52950.023.28669414
Produced by FastQC (version 0.11.5)