FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368694
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50210.21197334902693216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38970.16452103986416144No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34840.14708527146182665No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33010.13935949514795917No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGGTTCAGGGAGA32430.13691088844738913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32170.1358132371678233No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30840.1301983286992748No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30190.1274542005003601No Hit
GTCTATTACTGTCAACAATATGGAAGTTCTCCCCTAATCACCTTCGGCCA29350.12390794252022423No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATT29000.12243033502850093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27570.11639325299088864No Hit
GTCTCTGGTGGCTCCATCAGTGACACTGGGTACTACTGGAGCTGGATCCG26180.11052504038090188No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25460.10748539068364256No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24280.10250374256868976No Hit
GCATAAAACTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT24130.10187048221509405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.043.0897641
GTATCAA86000.041.2245671
TATCAAC98400.035.9604032
ATCAACG104250.034.107573
TCAACGC105100.033.8317224
GTGGTAT13250.033.2515141
CAACGCA107850.033.0648165
TGGTATC13600.032.396462
AACGCAG110550.032.28846
CGCAGAG120100.029.6841138
ACGCAGA121300.029.3627037
AGAGTAC122350.029.10948411
CAGAGTA125350.028.38535510
GCAGAGT127200.028.0536779
AATAGCG2550.027.52181262
AGTACGG77400.026.40849313
GAGTACG78500.026.16994712
ATAGCGG2700.026.0225163
ACCGATC3100.025.55020927
TACTGAT18550.024.56170370
Produced by FastQC (version 0.11.5)