FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154412
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.10455539270208555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.043.7729841
GTATCAA50850.037.2550351
TATCAAC56650.033.8035772
CTCGTAT2150.032.19769739
ATCAACG59800.032.0229533
TCAACGC60850.031.4140834
TCGTATG2450.031.11332140
CAACGCA62400.030.6337685
AACGCAG63100.030.2939326
CGTATGC2650.030.11752741
GTGGTAT7700.028.9183461
TGGTATC7700.028.9183462
CGCAGAG68750.027.795898
ACGCAGA68900.027.7862857
TCTCGTA2400.027.35846738
TATGCCG2950.027.10468943
AGAGTAC71950.026.55966211
GCCGTCT2900.026.4651546
CAGAGTA73000.026.31837710
GCAGAGT73550.025.9818849
Produced by FastQC (version 0.11.5)