FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154412
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26100.22608912589266225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10050.042.2594151
TGGTATC10150.039.44672
GTATCAA57400.038.3678051
GGTATCA24750.037.7794231
TATCAAC63750.034.4624142
ATCAACG67950.032.513993
TCAACGC68300.032.3707734
CAACGCA69400.031.8494155
AACGCAG70100.031.482646
ACGCAGA75300.029.308547
CGCAGAG77500.028.3443098
AGAGTAC78600.027.90417111
CAGAGTA80000.027.54395710
GCAGAGT80900.027.3219959
GAGTACG48150.024.55021312
AGTACGG47650.024.30698213
GAGTACT43400.023.61603412
CGAACTA1653.4033292E-922.84264429
AGTACTT44800.022.72649813
GTACTTT47000.021.66364314
Produced by FastQC (version 0.11.5)