FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977325
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42820.4381347044227867No Hit
GAGTTACACTACCCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40810.4175683626224644No Hit
ACACTACCCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGA35230.3604737421021666No Hit
GTGTAACTCTGTTGACAGTAGTAAGTCGCAGAATCTTCAGGTTGTAGACTGATGATGGTGAAAGTGAAAT34290.35085565190699103No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTCGCAGAATCTTCAGGTTGTAGACTGATGATGGTGAAAGTGAAAT33280.34052132095260024No Hit
GTCTACAACCTGAAGATTCTGCGACTTACTACTGTCAACAGAGTTACACTACCCCGTGGACGTTCGGCCACGGGA28730.2939656716036119No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27120.2774921341416622No Hit
GTTGGAGACAGCGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATAGTTATTTAAATTGGTATCAGCAGAAA25440.2603023559204973No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24780.2535492287621825No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24440.25007034507456577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23610.24157777607244263No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22670.23195968587726704No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22590.2311411250095925No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCCACGTTCAGTGGCAGTGGATCTGAGACAGATTTCA21030.21517918808993938No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCCACGTT20340.20811910060624667No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18820.19256644412043075No Hit
GTGGGGTCCCATCCACGTTCAGTGGCAGTGGATCTGAGACAGATTTCACT18680.19113396260200036No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18450.1887806001074361No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGCGTCACCATCACTT18290.18714347837208706No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18200.18622259739595326No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGAA18150.18571099685365666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17640.1804926713222316No Hit
GTAGTAAGTCGCAGAATCTTCAGGTTGTAGACTGATGATGGTGAAAGTGAAATCTGTCTCAGATCCACTGCCA16070.16442841429411914No Hit
ACCCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC16030.1640191338602819No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15500.1585961681119382No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15420.15777760724426368No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15230.1558335251835367No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14940.15286624203821655No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14310.14642007520527972No Hit
GTTGTAGACTGATGATGGTGAAAGTGAAATCTGTCTCAGATCCACTGCCA14180.14508991379530864No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14120.14447599314455273No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14060.14386207249379684No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14040.14365743227687822No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14040.14365743227687822No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCGTGGCCGAACGTC14010.14335047195150027No Hit
ATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCCACGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTTTCA13770.1408947893484767No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13510.13823446652853452No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13510.13823446652853452No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13110.1341416621901619No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCA13060.13363006164786534No Hit
GTCTGCATCTGTTGGAGACAGCGTCACCATCACTTGCCGGGCAAGTCAGA12430.1271838948149285No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12250.12534213286266083No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12170.12452357199498632No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12080.12360269101885249No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12050.12329573069347455No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11810.12084004809045097No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11810.12084004809045097No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11680.11950988668047989No Hit
ATCTGAGACAGATTTCACTTTCACCATCATCAGTCTACAACCTGAAGATT11370.11633796331824112No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11220.11480316169135138No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10960.1121428388714092No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10800.11050571713606017No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10620.10866395518379249No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10530.10774307420765866No Hit
GATCAGGAGTTTAGGGGCTTCCCCCGGTTTCTGCTGATACCAATTTAAAT10480.10723147366536209No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10330.10569667203847236No Hit
GCATAGATCAGGAGTTTAGGGGCTTCCCCCGGTTTCTGCTGATACCAATT10330.10569667203847236No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10160.103957230194664No Hit
TCCACGTTCAGTGGCAGTGGATCTGAGACAGATTTCACTTTCACCATCAT10140.10375258997774538No Hit
GGTTGTAGACTGATGATGGTGAAAGTGAAATCTGTCTCAGATCCACTGCC10110.10344562965236744No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10100.10334330954390812No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9990.10221778835085564No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9920.10150154759164046No Hit
CCTTGGTCCCGTGGCCGAACGTCCACGGGGTAGTGTAACTCTGTTGACAG9840.10068298672396593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3900.044.9252971
GTATCAA34900.042.1311871
TATCAAC39950.036.6240922
TCAACGC42250.034.1783454
ATCAACG42550.034.119553
CAACGCA43100.033.4854355
AACGCAG44000.032.8784146
CGCAGAG46650.030.937248
ACGCAGA47150.030.6091657
AGAGTAC47300.030.51209811
ACCGAAC450.004744904630.50304825
GCAGAGT47900.030.12999
CAGAGTA51050.028.53936610
CGCGACT500.00798065627.42324812
GAGTACG30650.027.2890412
AGTACGG30750.027.0915913
ATATACC907.223527E-626.6778724
CGAAATT809.0283815E-526.09377348
TACGGGA15400.025.83248515
GAGTACT23500.025.67282912
Produced by FastQC (version 0.11.5)