FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584379
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19440.12269791508218678No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18720.1181535478569206No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17760.1120943915565657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17200.10855988371469201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.10710821085106531No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16940.10691886221667922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9350.050.809111
TGGTATC11200.042.0851972
GTATCAA76800.041.821011
GGTATCA29150.040.5069581
TATCAAC85700.037.295952
TCAACGC91150.035.0194634
ATCAACG91500.034.9849173
CAACGCA93350.034.212595
AACGCAG94800.033.7958376
ACGCAGA100950.031.7029367
CGCAGAG102500.031.3910338
AGAGTAC104050.030.69142311
CAGAGTA105600.030.27440610
GCAGAGT106950.030.05289
TAAGCGA500.00789226227.48695820
GAGTACG67600.027.2765912
AGTACGG66900.027.15310113
GAGTACT54600.024.52643812
AGTACTT55100.024.11844313
GTACGGG75200.023.97576514
Produced by FastQC (version 0.11.5)