FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788877
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37120.4705423025389256No Hit
CTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG24400.3093004359361472No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23960.303722887091397No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23400.29662418856171496No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22640.2869902405571464No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA21560.27329989339275956No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21460.2720322686553164No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT21320.27025759402289584No Hit
GTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA21280.26975054412791855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21160.2682293944429867No Hit
GTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATT20810.26379270786193537No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20710.26252508312449213No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGG20280.2570742967534863No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA20120.2550460971735771No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19790.2508629355400145No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19640.24896149843384963No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19200.24338394958909942No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17880.22665130305484887No Hit
GTCTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGAC17480.22158080410507597No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAATCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA16340.2071298820982232No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16260.20611578230826857No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16000.2028199579909162No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15730.19939737119981948No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15270.19356629740758063No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCC14920.18912961082652938No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAAAGCGCAATGTCAGGGAGAACTGGTTCTTCGACGTGTCTACTGA14710.1864675988778986No Hit
CTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT14310.18139709992812567No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG14140.17924213787447218No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTC13340.1691011399749264No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCGCCC13190.16719970286876154No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13190.16719970286876154No Hit
GTCTCCACTCTCCCTGTCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCT13180.16707294039501722No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13110.16618560307880695No Hit
GTCTATTACTGTGCGAGAGATCGCACGTTCAGGGAGTACCCAAGAACCTA13060.16555179071008536No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12960.16428416597264212No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACT12460.15794604228542597No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12290.15579108023177252No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCCCTGA12130.15376288065186333No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCC12070.1530023058093974No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGA11870.15046705633451096No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11830.14996000643953367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11660.14780504438588019No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11650.14767828191213586No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11570.1466641821221813No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11510.14590360727971535No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11490.1456500823322267No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11480.14552331985848238No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAAAGT11430.14488950748976076No Hit
CCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAG11280.14298807038359593No Hit
CCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTG11260.14273454543610728No Hit
GCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGC11000.1394387211187549No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10900.13817109638131164No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10850.13753728401259005No Hit
ATCCATCACTATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCC10780.13664994669637978No Hit
CTCCCTGTCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTA10760.13639642174889116No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10500.13310059743153876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10200.12929772321920907No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTC10180.12904419827172042No Hit
GTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCC10150.12866391085048745No Hit
ATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCCGGCTCTCCAGGGGCGACGGACAGGGAGAGTGGAGA10060.12752304858678856No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10040.12726952363929991No Hit
AGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG9960.12625542384934535No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9830.12460751169066915No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9740.12346664942697023No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9590.1215652123208054No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9580.12143844984706106No Hit
CCACTCTCCCTGTCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGTAG9550.12105816242582809No Hit
GAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAA9540.1209313999520838No Hit
TTGTAGACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACTC9530.12080463747833946No Hit
CTTTGTAGACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT9420.11941025026715191No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9390.11902996284591895No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9060.1148468012123563No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9020.11433975131737902No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9010.11421298884363468No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8980.1138327014224017No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8980.1138327014224017No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8870.11243831421121418No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8770.11117068947377093No Hit
CTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCCCT8660.1097763022625834No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8580.10876220247262881No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT8500.10774810268267423No Hit
GTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAAAG8470.10736781526144126No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8460.10724105278769694No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8380.10622695299774235No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8190.10381846599660023No Hit
GCCCTGGCTTCTGCAGGTACCAAGCCAAATCGTTGTATCCATCACTATGC8160.10343817857536726No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8120.10293112868038996No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8110.10280436620664565No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8060.10217055383792405No Hit
TTTGTAGACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT8050.10204379136417971No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7980.10115645404796945No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7910.1002691167317592No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7890.10001559178427055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)