FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788877
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35230.44658419500124863No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21350.2706378814441288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21230.2691167317591969No Hit
CTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG21220.2689899692854526No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20820.2639194703356797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20530.26024335859709435No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA19710.24984883575005992No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19030.24122898753544597No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18860.23907402548179246No Hit
GTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA18540.23501762632197415No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18510.23463733890074118No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17780.22538367831740563No Hit
GTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATT17600.2231019537900078No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA17500.2218343290525646No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16520.20941160662562097No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16390.20776369446694481No Hit
GTCTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGAC16220.2056087324132913No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGG15990.20269319551717185No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15580.19749593409365465No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15540.19698888419867736No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14520.18405911187675644No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAATCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA14070.17835480055826192No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAAAGCGCAATGTCAGGGAGAACTGGTTCTTCGACGTGTCTACTGA13600.17239696429227877No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13580.17214343934479012No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13560.17188991439730147No Hit
CTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT13420.17011523976488097No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13220.1675799902899945No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13060.16555179071008536No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12790.16212920391898863No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCC12710.16111510412903407No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACT12490.15832632970665894No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12490.15832632970665894No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12410.15731222991670438No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12210.15477698044181792No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCGCCC12020.1523684934406758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11540.14628389470094832No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11490.1456500823322267No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTC11430.14488950748976076No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCC11390.1443824575947835No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11280.14298807038359593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10790.13677670917012413No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGA10500.13310059743153876No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10480.13284707248405012No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10470.1327203100103058No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10350.13119916032537393No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10250.1299315355879307No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCCCTGA10220.12955124816669772No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10100.12803009848176586No Hit
CCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTG10000.12676247374432262No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9920.12574837395436805No Hit
CTCCCTGTCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTA9910.12562161148062373No Hit
GTCTATTACTGTGCGAGAGATCGCACGTTCAGGGAGTACCCAAGAACCTA9830.12460751169066915No Hit
CCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAG9720.1232131244794816No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9700.12295959953199295No Hit
GTCTCCACTCTCCCTGTCCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCT9670.12257931211075998No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9540.1209313999520838No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAAAGT9430.11953701274089623No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9420.11941025026715191No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9410.1192834877934076No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTC9300.11788910058222005No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9110.1154806135810779No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9010.11421298884363468No Hit
TTGTAGACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACTC8840.11205802678998121No Hit
GTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCC8790.11142421442125959No Hit
CTTTGTAGACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT8660.1097763022625834No Hit
GTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAAAG8550.10838191505139584No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8530.10812839010390721No Hit
CTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCCTCCGGGGTCCCT8480.1074945777351856No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8480.1074945777351856No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG8480.1074945777351856No Hit
ATCCATCACTATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCC8400.10648047794523101No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8360.10597342805025371No Hit
GCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGC8250.10457904083906616No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG8240.10445227836532185No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8180.10369170352285591No Hit
TTTGTAGACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT8110.10280436620664565No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8100.10267760373290133No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8060.10217055383792405No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8050.10204379136417971No Hit
GCCCTGGCTTCTGCAGGTACCAAGCCAAATCGTTGTATCCATCACTATGC8030.10179026641669107No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8020.10166350394294674No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7920.10039587920550351No Hit
GAGTGGAGGCTGAGGATGTTGGGGTCTATTACTGCATGCAAGGTCTACAA7910.1002691167317592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)