FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790953
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.1094881743921573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6150.043.5740051
GTATCAA37450.038.7140541
TGGTATC7300.036.2389762
TCGTACG400.00234411635.22491562
TATCAAC42150.034.8047332
TCGTATG2000.034.62788440
GGTATCA16500.034.148211
ATCAACG44250.033.385913
TCAACGC44550.033.1610874
CAACGCA45300.032.6879045
AACGCAG45900.032.185766
CGTATGC2350.030.96182341
CTCGTAT1359.094947E-1230.75094839
ACGCAGA49850.029.6335627
CGCAGAG50900.029.0897568
ACGAGAC2800.028.22313722
AGAGTAC52350.028.15277311
CAGAGTA53250.027.67694910
GCAGAGT53550.027.52199
TATACAC2750.027.4851463
Produced by FastQC (version 0.11.5)