FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790953
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19190.24261871438631624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5150.041.36021
GTATCAA38900.040.2729451
GGTATCA15250.040.100361
TATCAAC42750.036.5471342
TGGTATC6200.034.8920822
TCAACGC45600.033.985374
ATCAACG45950.033.820373
CAACGCA46500.033.3170555
AACGCAG47950.032.4504626
CGTACGC450.00410533431.41833163
ACGCAGA50850.030.6671987
CGCAGAG52000.029.988988
AGAGTAC52350.029.52660411
CAGAGTA53150.029.21114310
GCAGAGT54150.028.6084029
CCGTCAT1406.261068E-527.53045870
GAGTACG33200.026.01453812
AGTACGG32950.025.79585513
TACGGGG13700.023.77956215
GTACGGG36350.023.29758614
Produced by FastQC (version 0.11.5)