FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763978
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19280.25236328794808227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34650.041.1755451
GTGGTAT5300.040.217061
GGTATCA14500.038.646851
ACGGGTA1009.094947E-1237.7377916
TATCAAC40200.035.3012962
TGGTATC6000.034.933752
ATCAACG42900.033.140083
CAACGCA43500.032.9598735
TCAACGC43300.032.8167274
AACGCAG45000.032.0136576
ACGCAGA47850.030.1068887
CGCAGAG48750.029.551078
AGAGTAC49850.028.69256811
GCAGAGT50450.028.4873059
CAGAGTA50400.028.44751210
GAGTACG30150.027.07800112
AGTACGG30500.026.87973613
TACGGGG14000.025.24021115
GGTTAAC851.4774513E-424.2676011
GTACGGG33700.023.92333814
Produced by FastQC (version 0.11.5)